Presentation Type
Poster
Full Name of Faculty Mentor
Brian M. Lee, Chemistry
Major
Chemistry
Presentation Abstract
Extracellular Vesicles, also referred to as EVs, are spherical lipid membrane-bound vesicles produced by both Gram positive and Gram negative bacteria. These vesicles are secreted into the extracellular space and play important functions in cellular and host communication, elimination of competitors, virulence, detoxification of environmental stress, and nutrition sensing. They are often packed with proteins, enzymes, lipids, and nucleic acids like DNA or RNA molecules among other biological entities. Streptococcus thermophilus is a lactic acid bacterium (LAB), inhabiting the human digestive tract, that has been shown to produce EVs. The bacterial flora has a great impact on the host immune system, metabolism, and neurological processes, however, not a lot is known about the biochemical pathways behind this impact. Since extracellular vesicles are involved in host communication, they play a key role in the impact that bacterial flora has on the biochemical processes of a host. Therefore S. Thermophilus was grown aerobically at 37° C in M17 media, two other LABs were grown including Lactobacillus acidophilus, and Lactobacillus bulgaricus. The extracellular vesicles will then be isolated through centrifugation, then the EVs content will be analyzed further. Size comparison can be conducted using gel electrophoresis, on various RNA molecules hypothesized to be held within the membranes of EVs. A previous study in this lab isolated the AsdS sRNA molecule, that is 152 base pairs in length, and is involved in quorum sensing. This gene is conserved among Streptococcus species and can be observed in S. pyogenes as the MarS. Since S. thermophilus is a non-pathogenic species the Asd gene cannot be involved in virulence as MarS is responsible for virulence in S. pyogenes. Based on functional predictions, AsdS is responsible for intraspecies communication, biofilm formation, and transport.
Location
Poster Session 1
Start Date
12-4-2022 12:30 PM
End Date
12-4-2022 2:30 PM
Disciplines
Chemistry
Recommended Citation
Busby, L. Brooke; Myers, Isabel; and Hoxha, Klea, "Isolation and Analysis of Extracellular Vesicles from Lactic Acid Bacteria" (2022). Undergraduate Research Competition. 12.
https://digitalcommons.coastal.edu/ugrc/2022/fullconference/12
Included in
Isolation and Analysis of Extracellular Vesicles from Lactic Acid Bacteria
Poster Session 1
Extracellular Vesicles, also referred to as EVs, are spherical lipid membrane-bound vesicles produced by both Gram positive and Gram negative bacteria. These vesicles are secreted into the extracellular space and play important functions in cellular and host communication, elimination of competitors, virulence, detoxification of environmental stress, and nutrition sensing. They are often packed with proteins, enzymes, lipids, and nucleic acids like DNA or RNA molecules among other biological entities. Streptococcus thermophilus is a lactic acid bacterium (LAB), inhabiting the human digestive tract, that has been shown to produce EVs. The bacterial flora has a great impact on the host immune system, metabolism, and neurological processes, however, not a lot is known about the biochemical pathways behind this impact. Since extracellular vesicles are involved in host communication, they play a key role in the impact that bacterial flora has on the biochemical processes of a host. Therefore S. Thermophilus was grown aerobically at 37° C in M17 media, two other LABs were grown including Lactobacillus acidophilus, and Lactobacillus bulgaricus. The extracellular vesicles will then be isolated through centrifugation, then the EVs content will be analyzed further. Size comparison can be conducted using gel electrophoresis, on various RNA molecules hypothesized to be held within the membranes of EVs. A previous study in this lab isolated the AsdS sRNA molecule, that is 152 base pairs in length, and is involved in quorum sensing. This gene is conserved among Streptococcus species and can be observed in S. pyogenes as the MarS. Since S. thermophilus is a non-pathogenic species the Asd gene cannot be involved in virulence as MarS is responsible for virulence in S. pyogenes. Based on functional predictions, AsdS is responsible for intraspecies communication, biofilm formation, and transport.