Date of Award

Spring 4-30-2025

Document Type

Thesis

Degree Name

Bachelor of Science (BS)

Department

Chemistry

College

College of Science

First Advisor

Paul E. Richardson

Abstract/Description

Bacteriophages, commonly referred to as phages, are non-living viruses that only infect bacteria. As the most abundant biological entities on Earth, understanding their role in regulating microbial populations is essential. This ongoing research aims to identify naturally occurring bacteriophages that could effectively control bacteria blooms. The primary objective of this study is to detect coliphages on Coastal Carolina University's campus and determine their viral families. Coliphages are a subgroup of bacteriophages that specifically infect coliform bacteria. Water samples were collected across multiple sites on campus, and polymerase chain reaction (PCR) was employed to amplify the viral DNA from the potential bacteriophages in the sample. Amplicons were visualized using gel electrophoresis as a tool to identify their viral families. This ongoing research has revealed seasonal variations in bacteriophage presence, with higher phage abundance typically observed in the spring. Coliphages were identified in in the main campus pond this spring semester which rarely yielded phage detection in the past. The continued detection of different viral families of bacteriophage, particularly Siphoviridae and Myoviridae, showcase this lab’s ability to identify infectious agents using molecular methods on the university’s campus. These viruses could be used to treat contaminated water sources, since these viruses are naturally occurring in our environment. Thus; these viruses can help keep our beaches open, help improve beach access, and increase tourist for our community.

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