Protocol to build a drug-testing pipeline using large populations of Drosophila melanogaster

Authors

• Jay DeLoriea, Coastal Carolina University
• Cassandra Millet-Boureima, Centre Universitaire de Santé McGill
• Chiara Gamberi, Coastal Carolina University

Document Type

Article

Publication Date

12-15-2023

Abstract

As a small animal that recapitulates many fundamental aspects of human disease, Drosophila lends itself to probing the biological activity of molecules and drug candidates. Here, we present a protocol to build a drug-testing pipeline in Drosophila. We describe steps for generating synchronous populations of Bicaudal C mutants by genetic crossing and wild-type fly culturing for controlled compound administration and exemplary phenotypic assays. For complete details on the use and execution of this protocol, please refer to Millet-Boureima et al.,¹ Millet-Boureima et al.,² and Gamberi et al.³

Comments

Cell Press originally published this article.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

DeLoriea J, Millet-Boureima C, Gamberi C. Protocol to build a drug-testing pipeline using large populations of Drosophila melanogaster. STAR Protoc. 2023 Dec 15;4(4):102747. doi: 10.1016/j.xpro.2023.102747. Available at https://digitalcommons.coastal.edu/biology/