Presentation Type

Poster

Full Name of Faculty Mentor

Michelle Barthet, Biology

Major

Biology

Presentation Abstract

Multiple forms of RNA exist in cells. The most common forms of RNA include tRNA, rRNA, and mRNA. LncRNAs are proposed to have multiple functions during seed development including gene silencing, organogenesis, and other molecular processes. LncRNAs are unknown or poorly understood because their importance has been questioned throughout early research. However, the lncRNA NTAB_LNC014148.1 is presumed to function in seed development of Nicotiana tabacum based on global gene expression studies. Bioinformatics analyses predicted that lncRNA NTAB_LNC014148.1 binds to at least two gene regions during embryonic development (gene regions Nitab4.5_0001672g0150.1 and Nitab4.5_0001972g0020.1). These two gene regions encode an Arf GTPase activating protein and oxidoreductase. ADP-ribosylation factor (Arf) is a type of small GTPase that acts as a carrier for vesicles during active transport and transports respective proteins through the Golgi complex. NADPH oxidoreductases are involved in the conduction of electron flow and generation, or prevention of reactive oxygen species (ROS) involved in cell signaling, cell death, and other aspects of cell development. Both the Arf GTPase and NADPH oxidoreductase are crucial proteins for seed germination. However, the exact role of lncRNA NTAB_LNC014148.1 in seed development is unknown. We aim to discern specifically where and when lncRNA NTAB_LNC014148.1 is expressed in N. tabacum seeds using RT-PCR as a first step in the characterization of this particular lncRNA. DNA: RNA interaction assays will be utilized to confirm interaction among lncRNA and predicted gene regions to further characterize lncRNA cellular function.

Start Date

11-4-2023 10:00 AM

End Date

11-4-2023 12:00 PM

Disciplines

Biology

Included in

Biology Commons

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Apr 11th, 10:00 AM Apr 11th, 12:00 PM

Specifying an identified lncRNAs modulation in cellular processes during early embryonic development of Nicotiana tabacum seedlings

Multiple forms of RNA exist in cells. The most common forms of RNA include tRNA, rRNA, and mRNA. LncRNAs are proposed to have multiple functions during seed development including gene silencing, organogenesis, and other molecular processes. LncRNAs are unknown or poorly understood because their importance has been questioned throughout early research. However, the lncRNA NTAB_LNC014148.1 is presumed to function in seed development of Nicotiana tabacum based on global gene expression studies. Bioinformatics analyses predicted that lncRNA NTAB_LNC014148.1 binds to at least two gene regions during embryonic development (gene regions Nitab4.5_0001672g0150.1 and Nitab4.5_0001972g0020.1). These two gene regions encode an Arf GTPase activating protein and oxidoreductase. ADP-ribosylation factor (Arf) is a type of small GTPase that acts as a carrier for vesicles during active transport and transports respective proteins through the Golgi complex. NADPH oxidoreductases are involved in the conduction of electron flow and generation, or prevention of reactive oxygen species (ROS) involved in cell signaling, cell death, and other aspects of cell development. Both the Arf GTPase and NADPH oxidoreductase are crucial proteins for seed germination. However, the exact role of lncRNA NTAB_LNC014148.1 in seed development is unknown. We aim to discern specifically where and when lncRNA NTAB_LNC014148.1 is expressed in N. tabacum seeds using RT-PCR as a first step in the characterization of this particular lncRNA. DNA: RNA interaction assays will be utilized to confirm interaction among lncRNA and predicted gene regions to further characterize lncRNA cellular function.

 

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