Presentation Type
Poster
Full Name of Faculty Mentor
Paul E. Richardson, Chemistry
Major
Biochemistry
Presentation Abstract
Human papillomavirus (HPV) is the most common sexually transmitted infection that accounts for approximately 5% of all cancers worldwide and affects more than 80 million people in the US alone, according to the CDC and National Cancer Institute. Human papillomaviruses are small, nonenveloped, icosahedral DNA viruses that infect squamous epithelial cells. The viral particles consist of a single double stranded DNA molecule bound to histones and contained within a protein capsid composed of structural proteins late (L)1 and L2. To date, over 100 different genotypes of HPV have been identified, and approximately 15 types are considered oncogenic in cervical, vulvar, vaginal, anal, penile squamous epithelia, and more recently, in head and neck squamous cells. This study aimed to develop an experimental methodology that will allow for the safe and effectual detection of HPV among members of Coastal Carolina University (CCU), Conway, South Carolina. Genomic isolation techniques were developed for the purpose of generating inactive noninfectious viral particles, while preserving its genomic fingerprint for future characterization. Bacteriophage T4 are highly robust viral particles that are found naturally in the external environment, serving as a model virus for the initial establishment of safe and effectual isolation techniques. Consensus primers were identified for a PCR-based HPV detection assay, targeting the conserved L1 and E6/E7 ORF regions of the HPV genome. The developed methodology provided effective and reproducible viral characterization, enabling the future applications of these techniques to be applied for the detection of HPV amongst members of the Coastal Carolina community.
Start Date
11-4-2023 10:00 AM
End Date
11-4-2023 12:00 PM
Disciplines
Biochemistry
Recommended Citation
Frantz, Nicole, "Developing a Safe and Effective Papillomavirus Screen to be Used on College Students" (2023). Undergraduate Research Competition. 18.
https://digitalcommons.coastal.edu/ugrc/2023/fullconference/18
Included in
Developing a Safe and Effective Papillomavirus Screen to be Used on College Students
Human papillomavirus (HPV) is the most common sexually transmitted infection that accounts for approximately 5% of all cancers worldwide and affects more than 80 million people in the US alone, according to the CDC and National Cancer Institute. Human papillomaviruses are small, nonenveloped, icosahedral DNA viruses that infect squamous epithelial cells. The viral particles consist of a single double stranded DNA molecule bound to histones and contained within a protein capsid composed of structural proteins late (L)1 and L2. To date, over 100 different genotypes of HPV have been identified, and approximately 15 types are considered oncogenic in cervical, vulvar, vaginal, anal, penile squamous epithelia, and more recently, in head and neck squamous cells. This study aimed to develop an experimental methodology that will allow for the safe and effectual detection of HPV among members of Coastal Carolina University (CCU), Conway, South Carolina. Genomic isolation techniques were developed for the purpose of generating inactive noninfectious viral particles, while preserving its genomic fingerprint for future characterization. Bacteriophage T4 are highly robust viral particles that are found naturally in the external environment, serving as a model virus for the initial establishment of safe and effectual isolation techniques. Consensus primers were identified for a PCR-based HPV detection assay, targeting the conserved L1 and E6/E7 ORF regions of the HPV genome. The developed methodology provided effective and reproducible viral characterization, enabling the future applications of these techniques to be applied for the detection of HPV amongst members of the Coastal Carolina community.