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Presentation Type
Presentation
Full Name of Faculty Mentor
Brian Lee and Gabriela Perez-Alvarado, Chemistry
Major
Biochemistry
Presentation Abstract
Streptococcus pyogenes, also known as group A Streptococcus (GAS) is associated with necrotizing fasciitis. The host immune response to this disease is inhibited by the exotoxin streptolysin S (SLS), which is encoded by the sagA gene. Disruption of a rho-independent terminator of the sagA gene leads to transcription of the full sag operon and releases SLS. The programs mfold and RNAfold were used to predict secondary structures of the terminator region. RNA transcripts were generated by in vitro transcription. Differential scanning fluorimetry was used to characterize the secondary structure of the terminator RNA and to select optimal conditions for 3D structure determination by X-ray crystallography. Three-dimensional homology models of the structural motifs within each construct were predicted using the FARFAR program within Rosetta. Determining the structure and interactions of the sagA terminator will add for the development of therapeutics that can decrease SLS expression.
Location
Virtual Session Room 1
Start Date
21-4-2021 5:30 PM
End Date
21-4-2021 5:50 PM
Recommended Citation
Nibar, Sara, "Regulatory RNA structure in Streptococcus pyogenes: Terminator of streptolysin S associated gene A" (2021). Undergraduate Research Competition. 20.
https://digitalcommons.coastal.edu/ugrc/2021/fullconference/20
Regulatory RNA structure in Streptococcus pyogenes: Terminator of streptolysin S associated gene A
Virtual Session Room 1
Streptococcus pyogenes, also known as group A Streptococcus (GAS) is associated with necrotizing fasciitis. The host immune response to this disease is inhibited by the exotoxin streptolysin S (SLS), which is encoded by the sagA gene. Disruption of a rho-independent terminator of the sagA gene leads to transcription of the full sag operon and releases SLS. The programs mfold and RNAfold were used to predict secondary structures of the terminator region. RNA transcripts were generated by in vitro transcription. Differential scanning fluorimetry was used to characterize the secondary structure of the terminator RNA and to select optimal conditions for 3D structure determination by X-ray crystallography. Three-dimensional homology models of the structural motifs within each construct were predicted using the FARFAR program within Rosetta. Determining the structure and interactions of the sagA terminator will add for the development of therapeutics that can decrease SLS expression.