Detection of Newly Synthesized Membrane Proteins in Isolated Squid Axoplasm from Loligo pealeii
Presentation Type
Event
Full Name of Faculty Mentor
Miguel Holmgren
Major
Marine Science
Minor
Mathematics-Applied
Presentation Abstract
Loligo pealeii, the longfin inshore squid, is used in many physiological studies because of its large-sized neurons and its ability to be easily isolated. We know that the cell body of the neuron contains the tools needed to synthesize proteins. However, it is unclear where these proteins are made within the axon, whether made local or transported there. This research is to determine if the isolated axoplasm contains the machinery to conduct protein synthesis. Samples were incubated with Shaker eGFP or Shaker myc RNA to for protein synthesis and stained with Alexa 647. Images were taken on the confocal microscope and analyzed with programming systems. It is likely that proteins are created locally within the isolated axoplasm. This is important because the surrounding membrane and Schwann cells that contain this machinery are not used in the isolated axoplasm itself.
Location
Lib Jackson Student Union, Atrium
Start Date
17-4-2019 4:30 PM
End Date
17-4-2019 6:30 PM
Disciplines
Oceanography
Recommended Citation
Rios-Brady, Lillian, "Detection of Newly Synthesized Membrane Proteins in Isolated Squid Axoplasm from Loligo pealeii" (2019). Undergraduate Research Competition. 42.
https://digitalcommons.coastal.edu/ugrc/2019/poster/42
Detection of Newly Synthesized Membrane Proteins in Isolated Squid Axoplasm from Loligo pealeii
Lib Jackson Student Union, Atrium
Loligo pealeii, the longfin inshore squid, is used in many physiological studies because of its large-sized neurons and its ability to be easily isolated. We know that the cell body of the neuron contains the tools needed to synthesize proteins. However, it is unclear where these proteins are made within the axon, whether made local or transported there. This research is to determine if the isolated axoplasm contains the machinery to conduct protein synthesis. Samples were incubated with Shaker eGFP or Shaker myc RNA to for protein synthesis and stained with Alexa 647. Images were taken on the confocal microscope and analyzed with programming systems. It is likely that proteins are created locally within the isolated axoplasm. This is important because the surrounding membrane and Schwann cells that contain this machinery are not used in the isolated axoplasm itself.