The Effects of Marine Natural Products on the Microenvironment of Pancreatic Cancer
Presentation Type
Event
Full Name of Faculty Mentor
Michael Pierce
Major
Biology
Minor
Marine Science
Presentation Abstract
Pancreatic cancer is the 4th leading cause of cancer deaths in the United States, with that number increasing each year. Two compounds from marine sponges—HB-131 and HB-018—were tested for potential anti-cancer properties. Conditioned media from PANC-1 cells was used for testing. A western blot determined matrix metalloproteinase (MMP) expression, while a zymogram determined MMP activity. A sandwich enzyme-linked immunosorbent assay (ELISA) array determined the effects of these compounds in 15 different cytokines. HB-131 did not inhibit any of the cytokines tested, which suggests that its inhibition of CCL-2 is specific and not caused by inhibiting protein synthesis. In addition to inhibiting degranulation, HB-018 appeared to downregulate the inflammatory cytokine IL-23, while upregulating the anti-inflammatory cytokine IL-10. HB-131 and HB-018 lowered MMP-2 activity, which might translate to lower metastatic activity. From these findings, HB-131 and HB-018 are one step closer to making it to clinical trials.
Course
BIOL 399
External Presentation
1
Location
Lib Jackson Student Union, Atrium
Start Date
17-4-2019 4:30 PM
End Date
17-4-2019 6:30 PM
Disciplines
Biology
Recommended Citation
Davis, Whitney, "The Effects of Marine Natural Products on the Microenvironment of Pancreatic Cancer" (2019). Undergraduate Research Competition. 12.
https://digitalcommons.coastal.edu/ugrc/2019/poster/12
The Effects of Marine Natural Products on the Microenvironment of Pancreatic Cancer
Lib Jackson Student Union, Atrium
Pancreatic cancer is the 4th leading cause of cancer deaths in the United States, with that number increasing each year. Two compounds from marine sponges—HB-131 and HB-018—were tested for potential anti-cancer properties. Conditioned media from PANC-1 cells was used for testing. A western blot determined matrix metalloproteinase (MMP) expression, while a zymogram determined MMP activity. A sandwich enzyme-linked immunosorbent assay (ELISA) array determined the effects of these compounds in 15 different cytokines. HB-131 did not inhibit any of the cytokines tested, which suggests that its inhibition of CCL-2 is specific and not caused by inhibiting protein synthesis. In addition to inhibiting degranulation, HB-018 appeared to downregulate the inflammatory cytokine IL-23, while upregulating the anti-inflammatory cytokine IL-10. HB-131 and HB-018 lowered MMP-2 activity, which might translate to lower metastatic activity. From these findings, HB-131 and HB-018 are one step closer to making it to clinical trials.