Immunohistochemical analysis of tissue hypoxia in developing gecko brains
Presentation Type
Event
Full Name of Faculty Mentor
Scott Parker
Major
Biology
Second Major
Marine Science
Presentation Abstract
Reptile eggs can experience unpredictable shifts in O2 availability during development. Decrease in O2 may occur due to nest flooding, close packing of eggs, and nest microenvironment. We investigated effects of hypoxia on brains of Chondrodactylus turneri, and Correlophus ciliatus geckos using immunohistochemistry and light microscopy. Hypoxia treatments were applied after 75% of embryonic development was completed. Regional hypoxia was induced by covering 80% of egg surface area with paraffin wax and atmospheric hypoxia was induced by incubating eggs at 8% O2. Eggs were sampled 24 h, 5 d, or 9 d after application of hypoxia treatments. Hypoxic regions of immunohistochemically-labeled brains were imaged using light microscopy. Embryo mass and developmental stage were quantified to assess effects of hypoxia on development. Immunohistochemical staining of gecko brains from both regional and atmospheric hypoxia treatments showed defined areas of O2 depletion. Developmental hypoxia also reduced rate of development and growth in mass.
Location
Brittain Hall, Room 101
Start Date
17-4-2019 2:50 PM
End Date
17-4-2019 3:10 PM
Disciplines
Biology
Recommended Citation
Liner, Tessa, "Immunohistochemical analysis of tissue hypoxia in developing gecko brains" (2019). Undergraduate Research Competition. 37.
https://digitalcommons.coastal.edu/ugrc/2019/oral/37
Immunohistochemical analysis of tissue hypoxia in developing gecko brains
Brittain Hall, Room 101
Reptile eggs can experience unpredictable shifts in O2 availability during development. Decrease in O2 may occur due to nest flooding, close packing of eggs, and nest microenvironment. We investigated effects of hypoxia on brains of Chondrodactylus turneri, and Correlophus ciliatus geckos using immunohistochemistry and light microscopy. Hypoxia treatments were applied after 75% of embryonic development was completed. Regional hypoxia was induced by covering 80% of egg surface area with paraffin wax and atmospheric hypoxia was induced by incubating eggs at 8% O2. Eggs were sampled 24 h, 5 d, or 9 d after application of hypoxia treatments. Hypoxic regions of immunohistochemically-labeled brains were imaged using light microscopy. Embryo mass and developmental stage were quantified to assess effects of hypoxia on development. Immunohistochemical staining of gecko brains from both regional and atmospheric hypoxia treatments showed defined areas of O2 depletion. Developmental hypoxia also reduced rate of development and growth in mass.