Presentation Title

Regulatory RNA structure in Streptococcus pyogenes: Terminator of streptolysin S associated gene A

Presentation Type

Presentation

Full Name of Faculty Mentor

Brian Lee and Gabriela Perez-Alvarado, Chemistry

Major

Biochemistry

Presentation Abstract

Streptococcus pyogenes, also known as group A Streptococcus (GAS) is associated with necrotizing fasciitis. The host immune response to this disease is inhibited by the exotoxin streptolysin S (SLS), which is encoded by the sagA gene. Disruption of a rho-independent terminator of the sagA gene leads to transcription of the full sag operon and releases SLS. The programs mfold and RNAfold were used to predict secondary structures of the terminator region. RNA transcripts were generated by in vitro transcription. Differential scanning fluorimetry was used to characterize the secondary structure of the terminator RNA and to select optimal conditions for 3D structure determination by X-ray crystallography. Three-dimensional homology models of the structural motifs within each construct were predicted using the FARFAR program within Rosetta. Determining the structure and interactions of the sagA terminator will add for the development of therapeutics that can decrease SLS expression.

Location

Room 1

Start Date

21-4-2021 5:30 PM

End Date

21-4-2021 5:50 PM

Disciplines

Biochemistry

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Apr 21st, 5:30 PM Apr 21st, 5:50 PM

Regulatory RNA structure in Streptococcus pyogenes: Terminator of streptolysin S associated gene A

Room 1

Streptococcus pyogenes, also known as group A Streptococcus (GAS) is associated with necrotizing fasciitis. The host immune response to this disease is inhibited by the exotoxin streptolysin S (SLS), which is encoded by the sagA gene. Disruption of a rho-independent terminator of the sagA gene leads to transcription of the full sag operon and releases SLS. The programs mfold and RNAfold were used to predict secondary structures of the terminator region. RNA transcripts were generated by in vitro transcription. Differential scanning fluorimetry was used to characterize the secondary structure of the terminator RNA and to select optimal conditions for 3D structure determination by X-ray crystallography. Three-dimensional homology models of the structural motifs within each construct were predicted using the FARFAR program within Rosetta. Determining the structure and interactions of the sagA terminator will add for the development of therapeutics that can decrease SLS expression.

https://digitalcommons.coastal.edu/ugrc/test1/test1track/72