Presentation Title

Use of Loom-Mediated Isothermal Amplification (LAMP) for the Detection of Tomato Mosaic Virus

Presentation Type

Presentation

Full Name of Faculty Mentor

Michelle Barthet, Biology

Major

Biology

Second Major

Chemistry

Presentation Abstract

Tomato Mosaic Virus (ToMV) is a rapidly spreading single-strand RNA virus that can quickly infect and destroy entire crop yields. As there is no cure for ToMV, early detection is key; the infected plants must be identified, isolated, and destroyed before the infection spreads. Our aim was to develop a testing strategy that not only had the specificity to detect ToMV but could also be implemented in the field and quickly determine infection. Prior to this study, the most common form of detection was by use of polymerase chain reaction. While accurate, this testing method is costly and takes time, leading to greater crop loss. Our method used Loop Mediated Isothermal Amplification (LAMP) with specifically designed primers to target the coding region of the viral RNA responsible for the production of coat proteins. We were able to identify infected plants in as little as five minutes and with limited equipment.

Location

Room 2

Start Date

22-4-2021 2:40 PM

End Date

22-4-2021 3:00 PM

Disciplines

Biology

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Apr 22nd, 2:40 PM Apr 22nd, 3:00 PM

Use of Loom-Mediated Isothermal Amplification (LAMP) for the Detection of Tomato Mosaic Virus

Room 2

Tomato Mosaic Virus (ToMV) is a rapidly spreading single-strand RNA virus that can quickly infect and destroy entire crop yields. As there is no cure for ToMV, early detection is key; the infected plants must be identified, isolated, and destroyed before the infection spreads. Our aim was to develop a testing strategy that not only had the specificity to detect ToMV but could also be implemented in the field and quickly determine infection. Prior to this study, the most common form of detection was by use of polymerase chain reaction. While accurate, this testing method is costly and takes time, leading to greater crop loss. Our method used Loop Mediated Isothermal Amplification (LAMP) with specifically designed primers to target the coding region of the viral RNA responsible for the production of coat proteins. We were able to identify infected plants in as little as five minutes and with limited equipment.

https://digitalcommons.coastal.edu/ugrc/test1/test1track/51