Presentation Title

Use of Loop-Mediated Isothermal Amplification (LAMP) for the Detection of Tomato Mosaic Virus

Presentation Type

Presentation

Full Name of Faculty Mentor

Michelle Barthet Parker, Biology

Major

Biology

Presentation Abstract

Tomato Mosaic Virus (ToMV) is a rapidly spreading single-strand RNA virus that can quickly infect and destroy entire crop yields. As there is no cure for ToMV, early detection is key; the infected plants must be identified, isolated, and destroyed before the infection spreads. Our aim was to develop a testing strategy that not only had the specificity to detect ToMV but could also be implemented in the field and quickly determine infection. Prior to this study, the most common form of detection was by use of polymerase chain reaction. While accurate, this testing method is costly and takes time, leading to greater crop loss. Our method used Loop Mediated Isothermal Amplification (LAMP) with specifically designed primers to target the coding region of the viral RNA responsible for the production of coat proteins. We were able to identify infected plants in as little as five minutes and with limited equipment. Currently, testing is underway to determine the limits of detection for this method. We are also determining if this method will retain specificity across various tomato species.

Location

Room 2 (BRTH 112)

Start Date

12-4-2022 5:30 PM

End Date

12-4-2022 5:50 PM

Disciplines

Biology

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Apr 12th, 5:30 PM Apr 12th, 5:50 PM

Use of Loop-Mediated Isothermal Amplification (LAMP) for the Detection of Tomato Mosaic Virus

Room 2 (BRTH 112)

Tomato Mosaic Virus (ToMV) is a rapidly spreading single-strand RNA virus that can quickly infect and destroy entire crop yields. As there is no cure for ToMV, early detection is key; the infected plants must be identified, isolated, and destroyed before the infection spreads. Our aim was to develop a testing strategy that not only had the specificity to detect ToMV but could also be implemented in the field and quickly determine infection. Prior to this study, the most common form of detection was by use of polymerase chain reaction. While accurate, this testing method is costly and takes time, leading to greater crop loss. Our method used Loop Mediated Isothermal Amplification (LAMP) with specifically designed primers to target the coding region of the viral RNA responsible for the production of coat proteins. We were able to identify infected plants in as little as five minutes and with limited equipment. Currently, testing is underway to determine the limits of detection for this method. We are also determining if this method will retain specificity across various tomato species.