Presentation Type
Poster
Full Name of Faculty Mentor
Megan Cevasco, Biology
Major
Biology
Presentation Abstract
The Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Sciences (SEA-PHAGES) program was formed to conduct research on bacteriophages, viruses that infect bacteria. Using the bacterial host Mycobacterium smegmatis, DuncansLeg was isolated from a soil sample found on August 27th, 2021, at Coastal Carolina University's Campus. The enriched isolate obtained from an environmental distillate was purified via picking a well-isolated plaque and amplified by making webbed plates in preparation for DNA extraction. This isolate's DNA was characterized and analyzed through restriction digests. Once we obtained enough lysate we were able to send in the samples to be sequenced and further reviewed by bioinformatic analyses. DuncansLeg is a temperate phage that is a part of the L3 subcluster with 75,593 base pairs.
Location
Poster Session 2
Start Date
13-4-2022 4:30 PM
End Date
13-4-2022 6:30 PM
Disciplines
Biology
Recommended Citation
Burbage, Kaitlynn; Hibionada, Edenborough Jr.; and Presnell, Kirsten, "Discovery and Investigation of Novel Bacteriophage DuncansLeg" (2022). Undergraduate Research Competition. 11.
https://digitalcommons.coastal.edu/ugrc/2022/fullconference/11
Included in
Discovery and Investigation of Novel Bacteriophage DuncansLeg
Poster Session 2
The Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Sciences (SEA-PHAGES) program was formed to conduct research on bacteriophages, viruses that infect bacteria. Using the bacterial host Mycobacterium smegmatis, DuncansLeg was isolated from a soil sample found on August 27th, 2021, at Coastal Carolina University's Campus. The enriched isolate obtained from an environmental distillate was purified via picking a well-isolated plaque and amplified by making webbed plates in preparation for DNA extraction. This isolate's DNA was characterized and analyzed through restriction digests. Once we obtained enough lysate we were able to send in the samples to be sequenced and further reviewed by bioinformatic analyses. DuncansLeg is a temperate phage that is a part of the L3 subcluster with 75,593 base pairs.