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Presentation Type
Presentation
Full Name of Faculty Mentor
Michelle Barthet, Biology
Major
Biology
Presentation Abstract
Tomato mosaic virus (ToMV) belongs to the genus Tobamovirus consisting of positive-strand RNA viruses. ToMV has limited tomato production globally. Current testing methods require expensive molecular equipment for high complexity molecular diagnostics and are limited to a laboratory environment. Novel ToMV primers targeted against ToMV genes 2 and 4 were created for detection of ToMV in tomato leaves and were checked by BLAST analysis in GenBank for non-specificity to other viruses. A modified RNA extraction protocol and a simplified RT-PCR protocol with SYBR green was used to amplify and detect ToMV in a manner for replication in a kitchen environment. An internal control was incorporated to confirm positive results and successful RNA extraction. Initial tests demonstrated the efficacy of the methodology. This RNA extraction and RT-PCR approach will help detect ToMV outside of the laboratory and lead to detection methods for various viruses when materials and facilities are lacking.
Location
Virtual Session Room 1
Start Date
21-4-2021 5:10 PM
End Date
21-4-2021 5:30 PM
Disciplines
Biology
Recommended Citation
Petraccione, Kaylee; Lehmann, Emma; and Tancini, Molly, "Detection of Tomato Mosaic Virus Using a Novel at Home RT-PCR Approach" (2021). Undergraduate Research Competition. 18.
https://digitalcommons.coastal.edu/ugrc/2021/fullconference/18
Detection of Tomato Mosaic Virus Using a Novel at Home RT-PCR Approach
Virtual Session Room 1
Tomato mosaic virus (ToMV) belongs to the genus Tobamovirus consisting of positive-strand RNA viruses. ToMV has limited tomato production globally. Current testing methods require expensive molecular equipment for high complexity molecular diagnostics and are limited to a laboratory environment. Novel ToMV primers targeted against ToMV genes 2 and 4 were created for detection of ToMV in tomato leaves and were checked by BLAST analysis in GenBank for non-specificity to other viruses. A modified RNA extraction protocol and a simplified RT-PCR protocol with SYBR green was used to amplify and detect ToMV in a manner for replication in a kitchen environment. An internal control was incorporated to confirm positive results and successful RNA extraction. Initial tests demonstrated the efficacy of the methodology. This RNA extraction and RT-PCR approach will help detect ToMV outside of the laboratory and lead to detection methods for various viruses when materials and facilities are lacking.