Date of Award

Fall 12-15-2011

Document Type

Thesis

Degree Name

Bachelor of Science (BS)

Department

Biology

College

College of Science

First Advisor

Doug Van Hoewyk

Abstract/Description

Plants that hyperaccumulate elements like selenium to potentially toxic levels may use proteasome complexes to reduce toxicity. The 26S proteasome complex may be a pathway that these plants take to rid themselves of selenium toxicity by destroying damaged proteins caused by selenium. A method to test this hypothesis is to use a hyperaccumulator of selenium Stanleya pinnata and nonhyperaccumulators Populous tremula and Arabdopsis thaliana to evaluate their proteasome abundance without and with selenium. To compare these species western blots were made to show the differences in proteasome abundance. Also to compare the amount of oxidized and an ubiquinated protein in samples without selenium versus with selenium a proteasome inhibitor MG132 was used in another set of samples. The results showed that selenium does upregulate the 26s proteasome complex in Stanleya Pinnata and Populous tremula but not in Arabdopsis thaliana.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Included in

Biology Commons

Share

COinS